Comparing Norgen Biotek's Total RNA Purification Kit and Qiagen's RNase A
When it comes to performing RNA extractions, there are many kits to choose from. Two popular RNA extraction kits are Norgen Biotek's Total RNA Purification Kit and Qiagen's RNase A.
Norgen Biotek's Total RNA Purification Kit is a convenient, ready-to-use kit that quickly and easily isolates and purifies total RNA from a variety of samples including cultured cells, tissues, plant, and animal. It utilizes Norgen's patented resin-based purification technology to provide superior results. It is extremely fast and requires no phenol-chloroform extractions and is also compatible with PCR-based applications.
Qiagen's RNase A is a ribonuclease enzyme that is ideal for the efficient removal of genomic DNA from RNA preparations. It works by hydrolyzing and cleaving the phosphodiester bonds between deoxyribonucleotides which initiates a degradation process of any contaminating DNA in the sample. The added advantage of using Qiagen's RNase A is that it is non-toxic, and allows for very high purity of the resulting RNA.
Both Norgen Biotek's Total RNA Purification Kit and Qiagen's RNase A have distinct advantages and are ideal for anyone looking to quickly and efficiently perform RNA extractions. Each kit offers unique features that make them well-suited for different applications. The Total RNA Purification Kit offers convenience and quickness to finish the extraction while RNase A offers a high degree of purity in the end product. Therefore, it is important to consider the specific goals of the extraction when choosing between these two options.